|
| Status |
Public on Oct 01, 2015 |
| Title |
armGFP, nmoP1 eye imaginal discs, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
armGFP, nmoP1 eye imaginal discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: third instar larvae (at 25°C)
genotype/variation: armGFP_nmoP1 mutant
tissue: eye imaginal disc
|
| Treatment protocol |
Approximately 500 eye imaginal discs were dissected in cold PBS, then sedimented by microcentrifugation. PBS was completely removed and discs were stored at -80C.
|
| Growth protocol |
Flies were allowed to lay eggs for 6-12 h. These developed at 25C until third intar larvae.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the mirVANATM miRNA isolaton kit (Ambion#AM1260) following manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
labeling was performed according to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed following Affymetrix protocol, chips were washed and stained using Fluidics protocol midiEuk2v3_450 on an Affymetrix GeneChip Fluidics Station 450
|
| Scan protocol |
Affymetrix scanner GeneChip 3000 7G was used to read the arrays
|
| Description |
Gene expression data from armGFP, nmoP1 third instar larvae eye imaginal discs
NP_B
|
| Data processing |
Pre-processing of data was performed using the rma function of the affy package and differential expression analysis using the limma package, both from Bioconductor (www.bioconductor.com)
|
| |
|
| Submission date |
Feb 28, 2012 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Veronica Munoz-Soriano |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Valencia
|
| Department |
Genetics
|
| Street address |
C/ Dr. Moliner, 50
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
46100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE36127 |
Expression data from Drosophila third instar larvae eye imaginal discs |
|
