|
| Status |
Public on Nov 19, 2012 |
| Title |
RPE/TNFa rep2 |
| Sample type |
RNA |
| |
|
| Source name |
RPE_TNFa
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ARPE-19
cell type: Retinal pigment epithelial (RPE) cells
treated with: recombinant cytokine TNFa
|
| Treatment protocol |
ARPE19 were stimulated basolaterally for 48h with 1) recombinant IFNg (200ng/ml) and/or recombinant TNFa (40ng/ml) or 2) CD3/CD28 activated T cells (2.5 T cells/RPE cell) with anti-IFNg (40ug/ml) and/or anti-TNFa (118ug/ml).
|
| Growth protocol |
The adult human RPE cell line ARPE-19 (American Type Culture Collection) was cultured in 6-well cell culture plates on Anopore 0.2 um membrane inserts (Nunc) at 37 oC with 10% CO2 >6 weeks in DMEM+10%FCS, until pigmented monolayers had formed. Then media was gradually changed to X-vivo 15 serum-free medium (Lonza BioWhittaker) prior to experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NucleoSpin RNA II kit (Macherey-Nagel)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard protocol, Affymetrix Core center, Rigshospitalet, Copenhagen, Denmark
|
| Scan protocol |
Standard protocol, Affymetrix Core center, Rigshospitalet, Copenhagen, Denmark
|
| Description |
SAMPLE 7
|
| Data processing |
Expression Console 1.1 (Affymetrix)
|
| |
|
| Submission date |
Mar 07, 2012 |
| Last update date |
Nov 19, 2012 |
| Contact name |
Carsten Faber |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Copenhagen
|
| Department |
Fac. of Health Science, ISIM
|
| Lab |
Panum Inst. 18.3
|
| Street address |
Blegdamsvej 3
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36331 |
Chemokine expression in retinal pigment epithelial cells in response to co-culture with activated T Cells |
|
