|
| Status |
Public on Dec 01, 2012 |
| Title |
Uri_iPS1 |
| Sample type |
RNA |
| |
|
| Source name |
iPS cells derived from human urine cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: pluripotent stem cells (iPSCs)
|
| Treatment protocol |
no treatments
|
| Growth protocol |
The primary urine cells were cultured in urine cell medium consisting of a 1:1 mixture of DMEM/F12 culture medium supplemented with 10% of fetal bovine serum (FBS, PAA), 0.1 mM nonessential amino acids (NEAA), 1 mM L-glutamax, 0.1 mM ß-mercaptoethanol and SingleQuot Kit CC-4127 REGM (Lonza). Neural stem cells were dissociated to small cluster or single cells to suspend in flasks containing neural growth medium as neural spheres(1:1 of DMEM/F12 supplemented with 1% N2 (Invitrogen) and Neurobasal medium supplemented with 2% B27 (Invitrogen) supplemented with 20ng/ml bFGF, 20ng/ml EGF). iPS cell maintained on matrigel by defined medium_TeSR.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared with RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and subjected to on-column DNase treatment. Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Amino Allyl labeling method, About 5 μg total RNA of each sample was used for labeling and array hybridization as the following steps: 1) Reverse transcription with by invitrogen Superscript ds-cDNA synthesis kit; 2) ds-cDNA labeling with NimbleGen one-color DNA labeling kit
|
| |
|
| Hybridization protocol |
Array hybridization, wash were performed according to NimbleGen’s recommended protocol
|
| Scan protocol |
Arrays were scanned using a GenePix 4000B scanner and the PMT settings were followed by NimbleGen array manual
|
| Description |
iPS cells derived from human urine cells_replicate1
|
| Data processing |
Scanned images (TIFF format) were then imported into NimbleScan software (version 2.5) for grid alignment and expression data analysis. Expression data were normalized through quantile normalization and the Robust Multichip Average (RMA) algorithm included in the NimbleScan software
|
| |
|
| Submission date |
Mar 09, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Guangjin Pan |
| E-mail(s) |
[email protected]
|
| Organization name |
Guangzhou Institute of Biomedicine and Health, CAS
|
| Street address |
Kaiyuan Street, Guangzhou Science district
|
| City |
Guanghou |
| State/province |
China |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL15338 |
| Series (1) |
| GSE36395 |
Efficient Generation of Integration-free and Engraftable induced Neural Stem Cells (iNSC) from Epithelial-like Cells in Human Urine |
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