|
| Status |
Public on Mar 10, 2012 |
| Title |
Control_7 |
| Sample type |
RNA |
| |
|
| Source name |
Keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cells: Keratinocytes from skin biopsies with no stimulation.
disease state: healthy
|
| Treatment protocol |
No treatment before extraction.
|
| Growth protocol |
The Biopsies were mechanically homogenized (Rotor/Stator homogeniser, PowerGen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNEasy Fibrous Tissue Midi Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Probe labelling was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and EBS-MP samples.
|
| |
|
| Hybridization protocol |
Hybridation was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and EBS-MP samples.
|
| Scan protocol |
Scanning was done using a GeneArray Scanner at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and EBS-MP samples.
|
| Description |
Gene expression data from non EBS subject, without any stimulation.
|
| Data processing |
The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix) and the raw image fils obtained (.CEL) were used for statistical analyses. Affy and Limma packages in Bioconductor (www.bioconductor.org) using R2.12.0 were used to perform quality tests, RMA normalization and Smyth's moderated t-test.
|
| |
|
| Submission date |
Mar 09, 2012 |
| Last update date |
Mar 10, 2012 |
| Contact name |
Catherine Laprise |
| Organization name |
Université du Québec à Chicoutimi
|
| Street address |
555 Boul. Université
|
| City |
Chicoutimi |
| State/province |
Qc |
| ZIP/Postal code |
G7H 2B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36406 |
Microarray analysis of Epidermolysis bullosa simplex-mottled pigmentation |
|
