|
| Status |
Public on Mar 22, 2012 |
| Title |
embryos from 2xbcd mother (control), biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila st12-13 embryo
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: TT16 males x TT16 female
tissue: dechorionated embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
we extracted RNA from more than 100 eggs by the RNeasy Kit (Qiagen) with subsequent DNase I digestion procedure
|
| Label |
biotin
|
| Label protocol |
We used the GeneChip One-Cycle Target Labeling and Control reagents (Affymetrix) for labeling
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
scanning were performed following Affymetrix technical manuals
|
| Description |
gene expression data in embryonic cell death
|
| Data processing |
The relative expression level of each probe set was analyzed by the Statistical Expression algorithm implemented in the GeneChip Operating System (GCOS, Affymetrix)
|
| |
|
| Submission date |
Mar 21, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kentaro M Tanaka |
| E-mail(s) |
[email protected]
|
| Organization name |
NIG
|
| Street address |
1111
|
| City |
Mishima |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE36675 |
Expression data from st 12-13 embryos from 2xbcd and 6xbcd mothers |
|
| Relations |
| Reanalyzed by |
GSE119084 |
