|
Status |
Public on Mar 28, 2012 |
Title |
MC0391_MCA0375_BM_CD138pos_GEP |
Sample type |
RNA |
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|
Source name |
MC0391_MCA0375_BM_CD138pos RNA
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD138 Positive Myeloma Cells sample order: 2 tc class: 6p21 cyto risk: low 17p13 deletion: no ploidy: hyperdiploid uams 70gene index: good copy number abnormalities: 11 time since sample1 months: 19.28
|
Treatment protocol |
CD138 positive tumor cells were isolated from bone marrow aspirates using magnetic sorting, both Miltenyi and Stem Cell Technologies
|
Growth protocol |
Bone marrow aspirates were attained from patients after they signed informed consent documetnation
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from cells using TRIzol (Invitrogen) in combination with PureLink Micro-to-Midi RNA columns (Invitrogen) using the manufacturers protocol. RNA samples were tested for purity and concentration on a Nanodrop spectrophotometer and quality checked on an Agilent Bioanalyzer
|
Label |
biotin
|
Label protocol |
2 ug of Total RNA was labeled using the One-Cycle Target Labeling kit from Affymetrix as per the manufacturers suggestion
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|
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Hybridization protocol |
Labeled cRNA was hybridized to U133Plus2.0 GeneChips in a GeneChip Hybridization Oven 640 and washed using a GeneChip Fluidics Station 450 as per the manufacturers protocol
|
Scan protocol |
Arrays were scanned using a GeneChip Scanner 3000 following the recommendations of the manufacturer
|
Description |
Single sample standard labeling and hybridization
|
Data processing |
Signal estimates were generated in Expression Console (Affymetrix) using the MAS5 algorithm with default settings
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|
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Submission date |
Mar 26, 2012 |
Last update date |
Mar 28, 2012 |
Contact name |
Jonathan J Keats |
E-mail(s) |
[email protected]
|
Phone |
602-343-8690
|
Organization name |
Translational Genomics Research Institute (TGen)
|
Department |
Integrated Cancer Genomics
|
Lab |
Multiple Myeloma Research Lab - Keats Lab
|
Street address |
445 N. Fifth Street
|
City |
Phoenix |
State/province |
Arizona |
ZIP/Postal code |
85004 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE36824 |
Clonal competition with alternating dominance in multiple myeloma [GEP] |
GSE36825 |
Clonal competition with alternating dominance in multiple myeloma |
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