|
| Status |
Public on Sep 21, 2012 |
| Title |
HCC70 shCont, replicate 1a |
| Sample type |
RNA |
| |
|
| Source name |
HCC70 breast cancer cell line, scrambled shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCC70
cell type: breast cancer cells
treatment: scrambled shRNA
|
| Treatment protocol |
Infected cells were exposed to viral supernatants of the specified vector in the presence of 6μg/ml polybrene. 48 hours post infection, cells were placed under 1μg/ml puromycin selection.
|
| Growth protocol |
Cells were grown under standard conditions as specified by the ATCC. 24 hours prior to RNA extraction, cells were seeded at equal confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Plus kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
200ng Total RNA were used. cDNA samples were generated and labeled using the Affymetrix WT-cDNA synthesis and labeling kit.
|
| |
|
| Hybridization protocol |
2ug ss cDNA in 80 ul were hybridized to a Human Gene 1.0 ST array using Affymetrix hybridization kit materials for 17 hours at 45° at 60rpm. Post-hybridization washes were done with Affymetrix wash solutions on the FS450 station using the FS450_0007 protocol.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
BP01
HCC70 cells stably infected with scrambled shRNA sequence.
|
| Data processing |
Data was quantified using Affymetrix Expression Console, applying the RMA method.
|
| |
|
| Submission date |
Mar 29, 2012 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Ittai Ben-Porath |
| E-mail(s) |
[email protected]
|
| Phone |
97226758150
|
| Organization name |
Hebrew University of Jerusalem
|
| Department |
Developmental Biology and Cancer Research
|
| Lab |
Ittai Ben-Porath
|
| Street address |
Faculty of Medicine, Ein kerem
|
| City |
Jerusalem |
| ZIP/Postal code |
Jerusalem 91120, Israel |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36939 |
EZH2 promotes a bi-lineage identity in basal-like breast cancer cells |
|
