|
| Status |
Public on Sep 24, 2012 |
| Title |
BM MSC1_late |
| Sample type |
RNA |
| |
|
| Source name |
BM MSC late
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Bone marrow derived mesenchymal stromal cells (MSC)
tissue origin: bone marrow
passage: 10
|
| Growth protocol |
Bone marrow fragments from tibia plateau of patients undergoing surgery were flushed with PBS. Cell suspension was filtered through a 100 µm cell strainer and centrifuged for 7 min at 400xg. Cells were seeded into 75 cm2 tissue culture flasks with DMEM-LG (PAA) with 10% FCS (PAA), 2 mM L-glutamine (Sigma-Aldrich) and 100 U/mL penicilline/streptomycine ( Lonza). The first medium exchange was performed after 48 hours to remove erythrocytes and other non-adherent cells. Thereafter, media was exchanged twice per week, and MSC were isolated by their plastic adherence. When reaching 80% confluence, cells were trypsinized, counted in a Neubauer counting chamber (Brand, Wertheim, Germany) and replated at 13,333 cells/cm2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mikroRNeasy kit (Quiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Arrays were scanned using Affymetrix GeneChip Scanner 3000.
|
| Data processing |
CEL files were processed using the Affymetrix Power Tools (APT) Software Package. Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
|
| |
|
| Submission date |
Apr 05, 2012 |
| Last update date |
Apr 12, 2013 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
[email protected]
|
| Phone |
+49 241 8088611
|
| Organization name |
RWTH Aachen University
|
| Department |
Helmholtz Institute for Biomedical Engineering
|
| Lab |
Stem Cell Biology and Cellular Engineering
|
| Street address |
Pauwelsstrasse 20
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (3) |
| GSE37065 |
Long-term culture associated gene expression changes in MSC [Affymetrix] |
| GSE37067 |
Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes |
| GSE46019 |
Long-term culture associated gene expression changes in MSC (TGFb treatment) |
|
