|
| Status |
Public on Mar 02, 2013 |
| Title |
met1-II_H3K9m2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
H3K9m2 ChIP on met1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col0
genotype/variation: met1 deletant
tissue: 3 wk-old leaves
chip antibody: H3k9m2 (Anti-Histone H3 (di methyl K9))
chip antibody vendor: Abcam
chip antibody cat. #: ab1220
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
As previously described (Bernatavichute et al., PLoS One. 2008 Sep 8;3(9):e3156.)
|
| Label |
Cy5
|
| Label protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| |
|
| Channel 2 |
| Source name |
H3 ChIP on met1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col0
genotype/variation: met1 deletant
tissue: 3 wk-old leaves
chip antibody: Anti-Histone H3
chip antibody vendor: Abcam
chip antibody cat. #: 1791-100
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
As previously described (Bernatavichute et al., PLoS One. 2008 Sep 8;3(9):e3156.)
|
| Label |
Cy3
|
| Label protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| |
|
| |
| Hybridization protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| Scan protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| Description |
Sample 2
|
| Data processing |
Each probe in the array was assigned a score define by the log2 ratio between Cy5 and Cy3 signal. The mean score of all probes in the array was substracted from the individual scores.
|
| |
|
| Submission date |
Apr 06, 2012 |
| Last update date |
Mar 03, 2013 |
| Contact name |
Hume Stroud |
| Organization name |
UT Southwestern
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL7143 |
| Series (2) |
| GSE37075 |
Loss of the DNA methyltransferase MET1 induces H3K9 hypermethylation at PcG target genes in Arabidopsis, and relocation of H3K27 trimethylation to transposons |
| GSE83606 |
Loss of the DNA methyltransferase MET1 induces H3K9 hypermethylation at PcG target genes in Arabidopsis, and relocation of H3K27 trimethylation to transposons [array] |
|
