|
| Status |
Public on Dec 10, 2012 |
| Title |
blastocyst [B96] |
| Sample type |
RNA |
| |
|
| Source name |
Cumulus cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Cumulus cells
oocyte developmental competence: B
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was carried out using the Rneasy Micro Kit (Qiagen). A DNase step was included in the extraction protocol to remove residual genomic DNA contamination.
|
| Label |
Cy5
|
| Label protocol |
RNA samples were prepared according to the Agilent protocol (Two-Color Microarray-162 Based Gene Expression Analysis) and hybridised on Whole Human Genome Oligo Microarray 4x44K (Agilent Technologies).
|
| |
|
| Hybridization protocol |
RNA samples were hybridised on Whole Human Genome Oligo Microarray 4x44K (Agilent Technologies: Two-Color Microarray-162 Based Gene Expression Analysis)
|
| Scan protocol |
Hybridized arrays were scanned using Agilent fluorescence scanner.
|
| Description |
Sample name: B96
Raw data file: 29_2.txt
|
| Data processing |
Hybridization and background signals were measured using GenePix Pro 5.0 image analysis software (Axon Instruments). For each microarray, Cy3 and Cy5 signal intensities were individually normalized to the median profile of all Cy3 or Cy5 signal intensities by Lowess
|
| |
|
| Submission date |
Apr 09, 2012 |
| Last update date |
Dec 10, 2012 |
| Contact name |
Raluca TEUSAN |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM U915
|
| Street address |
1, rue gaston veil
|
| City |
Nantes |
| ZIP/Postal code |
44035 |
| Country |
France |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE37116 |
Differentiating factors of cumulus cells related to quality of the human oocyte [CadoretPart3] |
| GSE37277 |
Differentiating factors of cumulus cells related to quality of the human oocyte |
|
