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Status
Public on Nov 03, 2006
Title
cr5_2_kg
Sample type
RNA
Channel 1 Source name
cr5_2_test
Organism
Saccharomyces cerevisiae Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0.1mg/ml'
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
cr5_2_ctrl
Organism
Saccharomyces cerevisiae Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0mg/ml'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at congo red (0.1mg/ml) vs non treated. 5 min from the start of treatment.
Data processing
VALUE=log(CH1/CH2)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH1/CH2))
Submission date
Jan 10, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected] URL
http://biopuce.insa-toulouse.fr Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250 Series (1)
GSE4049 Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions ID_REF VALUE normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN median in red channel
CH1_BKD_MEAN background in red channel
CH2_SIG_MEDIAN median in green channel
CH2_BKD_MEAN background in green channel
Data table ID_REF VALUE CH1_SIG_MEDIAN CH1_BKD_MEAN CH2_SIG_MEDIAN CH2_BKD_MEAN 01P09.2
0.134463
221
57
181
56
01P09.1
0.0670058
187
57
164
58
01P01.2
-0.246289
83
57
87
58
01P01.1
-0.00787783
90
57
87
58
01L17.2
0.398429
137
55
107
59
01L17.1
0.154617
140
57
124
62
01L09.2
-0.0940166
1245
59
1195
59
01L09.1
0.0699866
1833
58
1501
58
01L01.2
0.0170611
70
56
70
58
01L01.1
-0.13709
62
58
65
58
01H17.2
-0.174131
111
58
111
56
01H17.1
-0.319411
104
54
115
55
01H09.2
0.423223
414
55
259
54
01H09.1
0.342047
406
54
272
54
01H01.2
0.502776
257
56
161
55
01H01.1
0.465674
351
55
217
55
01D17.2
-0.19469
2323
63
2451
57
01D17.1
0.0157125
4507
61
3873
57
01D09.2
0.0584025
485
57
410
58
01D09.1
0.156013
428
58
334
58
15488 480 Kbytes .Supplementary data files not provided
