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Sample GSM916544 Query DataSets for GSM916544
Status Public on Dec 14, 2012
Title EBTPd12#2
Sample type RNA
 
Source name CD34+CD43+CD45+ cells/EBd12
Organism Homo sapiens
Characteristics cell type: CD34+CD43+CD45+ hematopoietic stem/progenitor cells
days of eb differentiation: 12
Growth protocol ES cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)-F12 (Sigma) supplemented with 1x MEM non-essential amino acids (Gibco-Invitrogen), 1x GlutaMAX-I (Gibco-Invitrogen), 20% knockout serum replacement (KSR) (Gibco-Invitrogen), 0.1 mM 2-mercaptoethanol (2-ME)(Sigma), and 5 ng/mL basic fibroblast growth factor (bFGF)(ReproCELL) either on irradiated MEF as feeder layers.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 45 μL of fragmented single-stranded DNA was mixed with 15 μL of master mix containing 5X TdT buffer, TdT and DNA Labeling reagent, and was incubated at 37°C for 60 minutes.
 
Hybridization protocol The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip ST cartridge. The arrays were incubated at 45°C for 17 hours ± 1 hour in a rotating oven at 60 rpm. The arrays were then washed and stained according to the GeneChip Expression Wash, Stain and Scan User Manual (P/N702731).
Scan protocol Fluorescence signals were scanned with the Affymetrix GeneChip Scaner 3000. Affymetrix GeneChip Command Console (AGCC) software was used for acquisition of the initial image of the scanned array.
Description CD34+CD43+CD45+ cells differentiated for 12 days in EBs
Data processing The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
 
Submission date Apr 17, 2012
Last update date Dec 14, 2012
Contact name Takaho A. Endo
E-mail(s) [email protected]
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
 
Platform ID GPL6244
Series (2)
GSE37348 Expression data of human ES cells-derived CD34+CD43+CD45low cells (hemogenic endothelium-like cells) expanded upon overexpression of Sox17
GSE38156 Critical role of SOX17 in the hematopoietic development from human embryonic stem cells

Data table header descriptions
ID_REF
VALUE log2 RMA-sketch signal.

Data table
ID_REF VALUE
7892501 5.157581858
7892502 6.975906379
7892503 5.029748034
7892504 9.970779407
7892505 5.742504086
7892506 6.452642395
7892507 6.234565958
7892508 7.410565991
7892509 11.85387476
7892510 5.122721313
7892511 5.147089446
7892512 8.541199419
7892513 4.453244709
7892514 11.40795828
7892515 10.33042862
7892516 5.708026967
7892517 7.723097789
7892518 4.352031793
7892519 6.094632131
7892520 10.10707985

Total number of rows: 32321

Table truncated, full table size 627 Kbytes.




Supplementary file Size Download File type/resource
GSM916544_RSM10483.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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