|
| Status |
Public on Apr 18, 2012 |
| Title |
yw-18hr_WithIR_2 |
| Sample type |
RNA |
| |
|
| Source name |
yw 18hr with IR sample 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: wing disc from 3rd instar larvae
genotype: y^1 w^1118
Sex: male and female
age: 3rd instar larvae
treatment: 18 hr with IR
|
| Treatment protocol |
2 and 18 hr with and without ionizing irradiation of larvae with 4000R of X-rays
|
| Growth protocol |
3rd instar larvae
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated from wing imaginal discs using the RNeasy Plus kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was biotin-labeled according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Biotin-labeled amplified RNA was fragmented and hybridized onto the microarrays (Drosophila GeneChip 2.0) according to the Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned using the Hewlett Packard Gene Array Scanner G2500A.
|
| Data processing |
The data were analyzed with the GCRMA method in R statistical environment 2.12.2.
|
| |
|
| Submission date |
Apr 18, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joseph K Heimiller |
| E-mail(s) |
[email protected]
|
| Organization name |
Univ of Colorado Boulder
|
| Department |
MCDB
|
| Street address |
347 UCB
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE37404 |
Ionizing Radiation-induced expression response in Drosophila Larvae |
|
| Relations |
| Reanalyzed by |
GSE119084 |
