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Status
Public on Nov 03, 2006
Title
cfw5_1_0704
Sample type
RNA
Channel 1 Source name
cfw5_1_ctrl2
Organism
Saccharomyces cerevisiae Characteristics
'drug: calcofluor white', 'time: 5 min', 'concentration: 0mg/ml'
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
cfw5_1_test2
Organism
Saccharomyces cerevisiae Characteristics
'drug: calcofluor white', 'time: 5 min', 'concentration: 0.02mg/ml'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at calcofluor white (0.02mg/ml) vs non treated. 5 min from the start of treatment.
Data processing
VALUE=log(CH2/CH1)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH2/CH1))
Submission date
Jan 11, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected] URL
http://biopuce.insa-toulouse.fr Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250 Series (1)
GSE4049 Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions ID_REF VALUE normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN median in red channel
CH1_BKD_MEAN background in red channel
CH2_SIG_MEDIAN median in green channel
CH2_BKD_MEAN background in green channel
Data table ID_REF VALUE CH1_SIG_MEDIAN CH1_BKD_MEAN CH2_SIG_MEDIAN CH2_BKD_MEAN 01P09.2
-0.0131128
153
52
170
63
01P09.1
-0.222052
112
55
119
70
01P01.2
0.0934819
85
57
100
67
01P01.1
-0.260063
88
59
90
66
01L17.2
-0.241779
125
55
128
69
01L17.1
-0.0914404
104
55
120
72
01L09.2
0.272497
1180
57
1647
64
01L09.1
0.195645
1366
56
1775
65
01L01.2
-0.0708212
60
54
71
68
01L01.1
0.0942586
98
59
117
71
01H17.2
0.0446917
102
53
117
62
01H17.1
-0.113381
103
55
111
65
01H09.2
-0.376914
256
55
217
69
01H09.1
-0.401281
255
56
210
67
01H01.2
-0.406131
228
56
188
65
01H01.1
-0.376631
223
56
187
64
01D17.2
0.375026
2332
59
3616
66
01D17.1
0.342071
1625
60
2444
79
01D09.2
-0.0546918
426
57
440
65
01D09.1
0.062361
413
55
473
64
15488 481 Kbytes .Supplementary data files not provided
