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Status
Public on Nov 03, 2006
Title
cr5_2_kr
Sample type
RNA
Channel 1
Source name
cr5_2_ctrl2
Organism
Saccharomyces cerevisiae
Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0mg/ml'
Extracted molecule
total RNA
Label
Cy5
Channel 2
Source name
cr5_2_test2
Organism
Saccharomyces cerevisiae
Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0.1mg/ml'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at congo red (0.1mg/ml) vs non treated. 5 min from the start of treatment.
Data processing
VALUE=log(CH2/CH1)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH2/CH1))
Submission date
Jan 11, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected]
URL
http://biopuce.insa-toulouse.fr
Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250
Series (1)
GSE4049
Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions
ID_REF
VALUE
normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN
median in red channel
CH1_BKD_MEAN
background in red channel
CH2_SIG_MEDIAN
median in green channel
CH2_BKD_MEAN
background in green channel
Data table
ID_REF
VALUE
CH1_SIG_MEDIAN
CH1_BKD_MEAN
CH2_SIG_MEDIAN
CH2_BKD_MEAN
01P09.2
-0.159945
312
65
243
85
01P09.1
-0.168627
188
65
162
84
01P01.2
-0.0808761
119
67
118
82
01P01.1
1.45
130
176
121
89
01L17.2
1.87608
166
161
131
82
01L17.1
-0.0683546
162
65
142
74
01L09.2
0.0182971
3277
67
2529
75
01L09.1
-0.095148
1941
67
1357
78
01L01.2
0.206806
79
66
87
75
01L01.1
0.286849
86
72
94
80
01H17.2
-0.775396
153
72
108
80
01H17.1
0.148012
121
67
120
73
01H09.2
0.0432266
332
73
274
71
01H09.1
-0.123838
352
64
259
68
01H01.2
-0.0564032
357
61
278
68
01H01.1
0.0157708
283
64
238
71
01D17.2
-0.0183392
5963
66
4418
72
01D17.1
0.112467
5184
66
4371
72
01D09.2
0.0492845
746
65
608
71
01D09.1
0.013428
737
64
581
69
Total number of rows: 15488 Table truncated, full table size 489 Kbytes .Supplementary data files not provided