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Status
Public on Nov 03, 2006
Title
cr5_1_kr
Sample type
RNA
Channel 1 Source name
cr5_1_ctrl2
Organism
Saccharomyces cerevisiae Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0mg/ml'
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
cr5_1_test2
Organism
Saccharomyces cerevisiae Characteristics
'drug: congo red', 'time: 5 min', 'concentration: 0.02mg/ml'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at congo red (0.02mg/ml) vs non treated. 5 min from the start of treatment.
Data processing
VALUE=log(CH2/CH1)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH2/CH1))
Submission date
Jan 11, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected] URL
http://biopuce.insa-toulouse.fr Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250 Series (1)
GSE4049 Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions ID_REF VALUE normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN median in red channel
CH1_BKD_MEAN background in red channel
CH2_SIG_MEDIAN median in green channel
CH2_BKD_MEAN background in green channel
Data table ID_REF VALUE CH1_SIG_MEDIAN CH1_BKD_MEAN CH2_SIG_MEDIAN CH2_BKD_MEAN 01P09.2
-0.273698
221
61
170
76
01P09.1
-0.0688122
210
63
178
72
01P01.2
0.258181
123
58
126
61
01P01.1
0.332289
98
59
102
60
01L17.2
-0.101821
145
59
120
60
01L17.1
-0.141618
144
56
118
59
01L09.2
0.18022
299
59
283
61
01L09.1
-0.0425554
3068
61
2289
63
01L01.2
0.184073
87
59
93
67
01L01.1
0.195661
90
57
94
63
01H17.2
-0.418159
119
60
93
63
01H17.1
-0.0295008
88
60
86
65
01H09.2
-0.105279
398
60
305
70
01H09.1
-0.161673
303
58
223
62
01H01.2
-0.129584
280
56
212
60
01H01.1
-0.120616
281
59
212
60
01D17.2
0.285586
4523
60
4646
59
01D17.1
0.176237
4049
58
3735
58
01D09.2
-0.0214471
802
60
620
59
01D09.1
0.0201652
837
58
674
60
15488 486 Kbytes .Supplementary data files not provided
