|
| Status |
Public on Jun 30, 2012 |
| Title |
Ada3FL/FL MEF, Cre, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Ada3FL/FL MEFs_Cre adenovirus
|
| Organism |
Mus musculus |
| Characteristics |
strain background: 129/B6 mixed
cell type: Ada3FL/FL MEF, immortalized
infected with: Cre adenovirus
|
| Treatment protocol |
Exponetially growing MEFs were infected with control or Cre adenovirus and total RNA was extracted from cells after 3 days of infection
|
| Growth protocol |
Ada3FL/FL MEFs were grown in DMEM medium supplemented with 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using Ambion Expression Kit according to the manufacturer's instructions from 200 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data of asynchronous cells after 3 days of Cre adenovirus infection
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using Robust Multi-chip Averaging (RMA) background correction as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 50.
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
Jun 30, 2012 |
| Contact name |
Vimla Band |
| Phone |
(402) 559-8565
|
| Organization name |
University of Nebraska Medical Center
|
| Department |
Genetics, Cell Biology and Anatomy
|
| Lab |
Vimla Band lab
|
| Street address |
Room 6011, DRC, 985805 Nebraska Medical Center
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198-5805 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE37542 |
Effect on gene expression upon deletion of Ada3 |
|
