|
| Status |
Public on Jul 18, 2012 |
| Title |
yeast recombinant line II-2B |
| Sample type |
genomic |
| |
|
| Source name |
yeast recombinant line II-2B
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
background strains: S96 and YJM 789
recombinant line: II-2B
|
| Growth protocol |
Yeast recombinant lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using Qiagen Genomic-tip 100/G columns, according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
Hybridized to Affymetrix Yeast S98 GeneChip(AYGC)
|
| Data processing |
Raw output CEL files were converted from compressed binary CEL files to CEL text files using Affymetrix CEL file conversion tool
(https://www.affymetrix.com/support/file_download.affx?onloadforward=/support/developer/downloads/Tools/CelFileConversion.ZIP).
CEL files were imported into Allelescan (http://med.stanford.edu/sgtc/research/download/) and data quality was determined
for parental and recombinant strains by using Allelescan’s quality check for controls, markers, hybridizations, and replicates.
Genotype Call: 0 (S96), 2 (YJM789) provided in the Series supplementary file
|
| |
|
| Submission date |
Apr 25, 2012 |
| Last update date |
Jul 18, 2012 |
| Contact name |
jianliang dai |
| E-mail(s) |
[email protected]
|
| Organization name |
Tulane University
|
| Department |
Tulane Center for Aging
|
| Street address |
1430 tulane ave
|
| City |
New Orleans |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE37590 |
Natural genetic variation in yeast longevity |
|
