|
| Status |
Public on Nov 01, 2012 |
| Title |
HEK-293 anti-miR-107 Experimental Replicate A 1521406093_C |
| Sample type |
RNA |
| |
|
| Source name |
HEK-293
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: anti-miR-107
cell line: HEK-293
|
| Treatment protocol |
Cells were transfected with either synthetic miRNA or anti-miR inhibitor oligonucleotides. Control refers to untransfected cell models; siEGFP control refers to transfection with an siRNA to EGFP as a transfection control.
|
| Growth protocol |
Cell models were grown as confluent monolayers in standard tissue culture conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with DNase I treatment and the RNeasy MinElute cleanup kit (Qiagen) in accordance with manufacturer's instructions. Quality control was performed with an Experion (BioRad) bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the TotalPrep RNA amplification kit (Ambion) for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Experimental Replicate A
|
| Data processing |
Illumina Beadstudio software computed average signal intensities and detection p-values.
|
| |
|
| Submission date |
May 02, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Adam Carroll |
| Organization name |
University of Newcastle
|
| Street address |
Ring Road
|
| City |
Callaghan |
| State/province |
NSW |
| ZIP/Postal code |
2308 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6098 |
| Series (1) |
| GSE37729 |
Genome-wide analysis of miRNA-associated transcriptome profiles in multiple cell models |
|
