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Sample GSM9270

Query DataSets for GSM9270

Status

Public on Sep 28, 2003

Title

ES cell line, RNA 70

Sample type

RNA

Source name

Abca1 +/- ES cell line

Organism

Mus musculus

Extracted molecule

total RNA

Description

ES cell line was grown on irradiated MEF feeders using standard culture conditions. Feeders were then depleted and ES cells plated on gelatin for ~2 days. RNA was isolated using Trizol/RNeasy, then hybridized to AffyMetrix mouse U74Av2 chip according to manufacturer's protocol. Data were normalized by AffyMetrix MAS5.0 using defaults.
Keywords = embryonic stem cholesterol transport

Submission date

Aug 28, 2003

Last update date

May 27, 2005

Contact name

Derek Symula

E-mail(s)

[email protected]

Phone

518-880-1317

Organization name

Wadsworth Center Genomics Institute

Street address

City

Troy

State/province

ZIP/Postal code

12180

Country

USA

Platform ID

GPL81

Series (1)

GSE614

effect of +/- mutations in ES cells

Data table header descriptions
ID_REF
VALUE	signal normalized by AffyMetrix MAS5.0
ABS_CALL	absolute call (Present, Marginal, Absent) per AffyMetrix MAS5.0

Data table
ID_REF	VALUE	ABS_CALL
100001_at	39	A
100002_at	162.3	A
100003_at	145.4	A
100004_at	1309.4	P
100005_at	3654.4	P
100006_at	69.4	A
100007_at	3203.8	P
100009_r_at	18887.4	P
100010_at	2063	P
100011_at	705.9	P
100012_at	2914	P
100013_at	105.9	A
100014_at	1201.5	P
100015_at	869.8	P
100016_at	939.5	P
100017_at	270	A
100018_at	760	A
100019_at	484.4	A
100020_at	992.3	P
100021_at	29.7	A

Total number of rows: 12488

Table truncated, full table size 218 Kbytes.

Supplementary data files not provided