|
| Status |
Public on Jun 08, 2013 |
| Title |
liver cells from wildtype 8wk male mouse 8 challenged with vehicle (saline)_8 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus liver - wildtype
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype/variation: wildtype
age: 8wks
developmental stage: adult
gender: male
agent: vehicle (saline)
tissue: liver
|
| Treatment protocol |
Mice were treated with either saline or 100mg/kg APAP by IP injection, liver samples harvested eight hours later.
|
| Growth protocol |
Experimental livers from male mice with a conditional-null allele of txnrd1 disrupted by albCre.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion RiboPure extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
One cycle biotinyllated cRNA generated from 1 µg total RNA with Ambion Message Amp-II Biotin Enhanced system.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix 7G scanner
|
| Description |
Gene expression data mus musculus liver tissue
PBS_8_++
|
| Data processing |
The data were imported into FlexArray using RMA preprocessing for analysis.
|
| |
|
| Submission date |
May 09, 2012 |
| Last update date |
Jun 08, 2013 |
| Contact name |
Ed Schmidt |
| E-mail(s) |
[email protected]
|
| Phone |
406 994-6375
|
| Organization name |
Montana State University
|
| Department |
M&I Dept
|
| Street address |
PO Box 173520
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE37874 |
Shifted Metabolic Bias in Livers of Mice Lacking Hepatocytic Thioredoxin Reductase-1 Protects Against Acetaminophen Toxicity |
|
