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Sample GSM929466 Query DataSets for GSM929466
Status Public on May 02, 2013
Title Control Day 1, Sample.11, miRNA
Sample type RNA
 
Source name Heart tissue, control, day 1
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
tissue: heart
treatment: vehicle control
dose: 0 ug
time point: day 1
sampleID: 343
Treatment protocol Female C57BL/6 mice (Taconic, Denmark) were acquired at 5-6 weeks of age and given food (Altromin no 1324; Christian Petersen, Denmark) and water ad libitum. Printex 90 was suspended by sonication in 0.9% NaCl MilliQ water containing 10% v/v acellular bronchial alveolar lavage fluid from C57BL/6 mice. A 40 µl suspension was instilled followed by 150 μl air.
Extracted molecule total RNA
Extraction protocol Total RNA enriched with small RNA was extracted using the mirVana Isolation kit (Ambion, Canada).
Label Cy3
Label protocol Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
 
Hybridization protocol Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
Scan protocol Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
Description Sample.11
Data processing The log2 of median signal intensites were cyclic lowess normalized using R software and the technical replicates were averaged using the median.
 
Submission date May 09, 2012
Last update date May 02, 2013
Contact name Andrew Williams
Organization name Health Canada
Street address 50 Columbine
City Ottawa
State/province ON
ZIP/Postal code K1A OK9
Country Canada
 
Platform ID GPL15547
Series (2)
GSE37890 Cardiac miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles
GSE37891 Cardiac gene and miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

Data table header descriptions
ID_REF
VALUE Median of the cyclic lowess-normalized log2 signal intensity

Data table
ID_REF VALUE
mcmv-miR-m01-1 5.605881703
mcmv-miR-m01-2 6.366163815
mcmv-miR-m01-2* 5.585728113
mcmv-miR-m01-3 5.687639313
mcmv-miR-m01-3* 5.568548107
mcmv-miR-m01-4 5.561739579
mcmv-miR-m01-4* 5.635700311
mcmv-miR-m107-1-3p 5.570238866
mcmv-miR-m107-1-5p 5.764407497
mcmv-miR-m108-1 5.585097232
mcmv-miR-m108-1* 5.772845248
mcmv-miR-m108-2-3p 5.582752967
mcmv-miR-m108-2-5p.1 5.65647213
mcmv-miR-m108-2-5p.2 5.576861559
mcmv-miR-m21-1 5.644803538
mcmv-miR-m22-1 5.782723993
mcmv-miR-M23-1-3p 5.96719292
mcmv-miR-M23-1-5p 6.938861271
mcmv-miR-M23-2 5.606092078
mcmv-miR-M23-2* 5.689451093

Total number of rows: 1136

Table truncated, full table size 28 Kbytes.




Supplementary file Size Download File type/resource
GSM929466_US22502706_253543010059_S01_miRNA_107_Sep09_1_3.txt.gz 2.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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