|
| Status |
Public on May 02, 2013 |
| Title |
High Dose Day 3, Sample.30, miRNA |
| Sample type |
RNA |
| |
|
| Source name |
Heart tissue, high-dose Printex 90, day 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
tissue: heart
treatment: Printex 90 carbon black nanoparticles
dose: 162 ug
time point: day 3
sampleID: 331
|
| Treatment protocol |
Female C57BL/6 mice (Taconic, Denmark) were acquired at 5-6 weeks of age and given food (Altromin no 1324; Christian Petersen, Denmark) and water ad libitum. Printex 90 was suspended by sonication in 0.9% NaCl MilliQ water containing 10% v/v acellular bronchial alveolar lavage fluid from C57BL/6 mice. A 40 µl suspension was instilled followed by 150 μl air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA enriched with small RNA was extracted using the mirVana Isolation kit (Ambion, Canada).
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
|
| |
|
| Hybridization protocol |
Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
|
| Description |
Sample.30
|
| Data processing |
The log2 of median signal intensites were cyclic lowess normalized using R software and the technical replicates were averaged using the median.
|
| |
|
| Submission date |
May 09, 2012 |
| Last update date |
May 02, 2013 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15547 |
| Series (2) |
| GSE37890 |
Cardiac miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles |
| GSE37891 |
Cardiac gene and miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles |
|
