|
| Status |
Public on May 22, 2012 |
| Title |
Stanford_ChipSeq_H1-hESC_SIN3A_(NB600-1263)_IgG-rab |
| Sample type |
SRA |
| |
|
| Source name |
H1-hESC
|
| Organism |
Homo sapiens |
| Characteristics |
lab: Stanford
lab description: Snyder - Stanford University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: H1-hESC
cell organism: human
cell description: embryonic stem cells
cell karyotype: normal
cell lineage: inner cell mass
cell sex: M
treatment: None
treatment description: No special treatment or protocol applies
antibody: SIN3A_(NB600-1263)
antibody antibodydescription: Rabbit polyclonal, Immunogen: Synthetic peptide: MKRRLDDQESDVYAAQQRR, corresponding to amino acids 1-19 of Mouse mSin3A. Antibody Target: SIN3A (NB600-1263)
antibody targetdescription: Mammalian Sin 3 (mSin 3) is closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
antibody vendorname: Novus Biologicals
antibody vendorid: NB600-1263
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
controlid: wgEncodeEH001834
replicate: 1
|
| Biomaterial provider |
WiCell Research Institute
|
| Treatment protocol |
None
|
| Growth protocol |
H1_ES_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
[email protected]
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX150369 |
| BioSample |
SAMN01000829 |
| Named Annotation |
GSM935289_hg19_wgEncodeSydhTfbsH1hescSin3anb6001263IggrabSig.bigWig |
