|
| Status |
Public on Nov 09, 2012 |
| Title |
b66+ |
| Sample type |
RNA |
| |
|
| Source name |
Human colon biopsies from UC patient with active disease (involved mucosa)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age (years): 47
evolution time (years): 1
disease extension: Left-sided colitis
treatment: Azathioprine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Rneasy Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
Gene expression data from human colon biopsies; involved area UC active patient
|
| Data processing |
The data were analyzed with Bioconductor tools in R (http://www.r-project.org) using GC-RMA as normalization method. Next we employ a conservative probe-filtering step excluding those probe sets not reaching a log2 expression values of 5 in at least 1 sample.
|
| |
|
| Submission date |
Jun 14, 2012 |
| Last update date |
Nov 10, 2012 |
| Contact name |
Azucena Salas |
| E-mail(s) |
[email protected]
|
| Organization name |
IDIBAPS
|
| Department |
Gastroenterology
|
| Street address |
Rosselló, 149-153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE38713 |
Expression data from intestinal mucosa of patients with UC |
|
