|
| Status |
Public on Jun 15, 2012 |
| Title |
fun30∆ transcritome replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
S288C background, rich medium, exponential phase
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4743
|
| Growth protocol |
Rich medium (YPD), exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard yeast protocol involving mechanical cell lysis with glass beads and vortex in the presence of phenol chloroform isoamyl alcohol (24:24:1). For details see Chanfreau et al. EMBO 1998.
|
| Label |
CY3
|
| Label protocol |
cDNA random priming using CyScribe lebelling kit from Amersham.
|
| |
|
| Channel 2 |
| Source name |
S288C background, rich medium, exponential phase
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: fun30 homozygote diploid from the systematic deletion library
|
| Growth protocol |
Rich medium (YPD), exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard yeast protocol involving mechanical cell lysis with glass beads and vortex in the presence of phenol chloroform isoamyl alcohol (24:24:1). For details see Chanfreau et al. EMBO 1998.
|
| Label |
CY5
|
| Label protocol |
cDNA random priming using CyScribe lebelling kit from Amersham.
|
| |
|
| |
| Hybridization protocol |
Breifly, DNA arrays were prehybridized using DIG Easy Hyb (Roche Applied Science) and single stranded DNA for 2 hours at 42 Celcius degrees, prior to over night hybridization in similar conditions. Two washes in with 0.1X SSC and 0.1% SDS were performed followed by one wash in with 0.1X SSC only.
|
| Scan protocol |
Scans of the microarrays were done on a GenePix 4000B scanner using the GenePix Pro software.
|
| Data processing |
print−tip Lowess normalisation using Goulphar R script ( Lemoine et al, BMC Bioinformatics, 2006)
|
| |
|
| Submission date |
Jun 14, 2012 |
| Last update date |
Jun 15, 2012 |
| Contact name |
bertrand llorente |
| E-mail(s) |
[email protected]
|
| Organization name |
cnrs
|
| Street address |
31 chemin josephh aiguier
|
| City |
marseille |
| ZIP/Postal code |
13009 |
| Country |
France |
| |
|
| Platform ID |
GPL1265 |
| Series (1) |
| GSE38735 |
Transcritome in the absence of FUN30 |
|
