|
| Status |
Public on Sep 24, 2012 |
| Title |
iPSC_donor5 cloneA |
| Sample type |
RNA |
| |
|
| Source name |
iPSC reprogrammed from MSC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue origin: bone marrow
cell type origin: mesenchymal stromal cells (MSC)
cell type: induced pluripotent stem cells (iPSC)
passage: p9
|
| Growth protocol |
Established iPSC colonies were maintained on MEFs in DMEM/F12 medium supplemented with Glutamax, 20% knockout serum replacer, 1% NAA, 0.1 mM βME and 50 ng/ml bFGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Quiagen)
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned using Affymetrix GeneChip Scanner 3000.
|
| Data processing |
CEL files were processed using the Affymetrix Power Tools (APT; version apt-1.12.0) Software Package. Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
|
| |
|
| Submission date |
Jun 19, 2012 |
| Last update date |
Sep 25, 2012 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
[email protected]
|
| Phone |
+49 241 8088611
|
| Organization name |
RWTH Aachen University
|
| Department |
Helmholtz Institute for Biomedical Engineering
|
| Lab |
Stem Cell Biology and Cellular Engineering
|
| Street address |
Pauwelsstrasse 20
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE37067 |
Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes |
| GSE38806 |
Gene expression profiles of induced pluripotent mesenchymal stromal cells [Affymetrix] |
|
