|
| Status |
Public on Dec 01, 2012 |
| Title |
HBV-associated acute liver failure liver from patient 32-3 |
| Sample type |
RNA |
| |
|
| Source name |
specimen obtained from explanted liver Patient 32
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: HBV-associated acute liver failure
subject: Patient 32
tissue: liver explant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 50 ng total RNA (GeneChip Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 to 18 µg (mean 12.8) of cRNA hybridized for 16 hr at 45°C on Affymetrics Human U133 Plus 2 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000_7Gplus
|
| Description |
Gene expression data from liver
|
| Data processing |
Affymetrics probeset summaries were computed using RMA algorithm. Data were then imported to BRB-ArrayTools and subsequent data filtering, median normalization and statistical analyses were performed using this software.
|
| |
|
| Submission date |
Jun 26, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Patrizia Farci |
| E-mail(s) |
[email protected]
|
| Phone |
301-5942325
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Allergy and Infectious Diseases
|
| Lab |
Hepatic Pathogenesis Section-Laboratory of Infectious Diseases
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-8009 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE38941 |
Liver Regeneration Gene Signature in Hepatitis B virus (HBV)-Associated Acute Liver Failure Identified by Gene Expression Profiling |
|
| Relations |
| Reanalysis of |
GSM366063 |
