|
| Status |
Public on Mar 07, 2006 |
| Title |
H1299p53+ hypoxia 24hrs rep1 |
| Sample type |
RNA |
| |
|
| Source name |
H1299 p53 wildtype cells exposed to hypoxia 24 hrs
|
| Organism |
Homo sapiens |
| Characteristics |
Cell Line: H1299, p53 reintroduced
Cell type: non-small cell lung cancer
|
| Treatment protocol |
Cells in monolayer culture were exposed to <0.01% oxygen in an anoxic incubator for the indicated times.
|
| Growth protocol |
Standard monolayer culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Affymetrix Genechip protocol
|
| Label |
biotin - streptavidin phycoerythrin (Affymetrix)
|
| Label protocol |
Affymetrix Genechip protocol
|
| |
|
| Hybridization protocol |
Affymetrix Genechip protocol
|
| Scan protocol |
Affymetrix Genechip protocol
|
| Description |
Affymetrix Genechip protocol
|
| Data processing |
Affymetrix Microarray Suite version 4
|
| |
|
| Submission date |
Feb 03, 2006 |
| Last update date |
Feb 08, 2006 |
| Contact name |
Nicholas Denko |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Radiation Oncology
|
| Street address |
Rm 1245 269 campus dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL3423 |
| Series (1) |
| GSE4186 |
Hypoxic changes in gene expression: effect of vhl and p53 genotype |
|
