|
| Status |
Public on Dec 21, 2012 |
| Title |
Tick-infested mouse, 12 hour after tick attachment, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BALB/cJ mouse ear, 4mm biopsy punch
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/cJ
stress: tick infestation
time: 12 hr
tissue: Skin
|
| Treatment protocol |
Mice were anesthetized and infested with 8 Ixodes scpularis nymphs. Attachment was monitored visually. At the proper times after attachment, mice were euthanized and 4mm biopsy punches from tick attachment sites were collected in RNALater (Ambion).
|
| Growth protocol |
Mice were housed at the University of Texas Medical Branch ABSL-2 animal facility, and fed with food and water ad libitum. All experiments were approved by the UTMB IACUC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a combination of Trizol and Rneasy microkit protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Ambion MessageAmp™ Premier RNA Amplification Kit from 500nanograms total RNA.
|
| |
|
| Hybridization protocol |
Total fragmented cRNA (10micrograms) was hybridized to the Affymetrix Mouse GeneChip 430A 2.0 array according to the manufacturer’s (Affymetrix, CA) conditions.
|
| Scan protocol |
Fluorescence was detected with an Affymetrix-7G Gene Array scanner using the Affymetrix GeneChip Command Console software (AGCC1.1).
|
| Description |
Gene expression from mice infested for 12 hrs
|
| Data processing |
Gene expression changes were identified using Partek Genomics Suite (Partek, MO) following the default gene expression workflow.
|
| |
|
| Submission date |
Jul 04, 2012 |
| Last update date |
Dec 21, 2012 |
| Contact name |
Dar Heinze |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Texas Medical Branch
|
| Department |
Pathology
|
| Street address |
301 University Blvd
|
| City |
Galveston |
| State/province |
Texas |
| ZIP/Postal code |
77555 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE39100 |
Early immunologic events at the tick-host interface |
|
