|
| Status |
Public on Jan 11, 2013 |
| Title |
IL8.2 |
| Sample type |
RNA |
| |
|
| Source name |
IL8 human transgenic mouse colon cancer tumor (control)
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: CBA/C57BL/6
genotype/variation: IL8 transgenic
tissue: colon cancer tumor
|
| Growth protocol |
Mice were maintained at all times on a 12-hour light-dark cycle in a specific pathogen-free environment with free access to rodent chow and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
12 μg of RNA dissolved in 300 μL of buffer was hybridized to Mouse Genome 430. 2.0 GeneChips (Affymetrix). All samples were prepared with the standard protocol recommended by the manufacturer.
|
| Scan protocol |
Arrays were scanned on an Affymetrix Scanner 3000-7G scanner with GCOS software. Scanning was peformed according to the protocol described in the Affymetrix GeneChip® Expression Analysis Technical Manual, November 2004 Edition.
|
| Data processing |
Data was normalized using the GCRMA algorithm as implemented in Bioconductor/R.
|
| |
|
| Submission date |
Jul 11, 2012 |
| Last update date |
Jan 11, 2013 |
| Contact name |
Timothy Cragin Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
Columbia University
|
| Department |
Medicine- Digestive and Liver Diseases
|
| Lab |
Wang Lab
|
| Street address |
1130 St. Nicholas Av
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE39273 |
Effect of transgenic human IL8 on gene expression in mouse colon cancer tumors |
|
