|
| Status |
Public on Oct 18, 2012 |
| Title |
WT AL eWAT biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild-type
genetic background: C57Bl/6J
age: 3 months
treatment: fed ad libitum
tissue: epididymal white adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
biological replicate 1
7539447027_C
|
| Data processing |
Image data were converted into unnormalized Sample Probe Profiles using the Illumina BeadStudio software and processed using the Model-Based Background Correction method (Xie, Wang, & Story, 2009). All gene expression values were log2 transformed and quantile normalized. Significance Analysis of Microarrays (Tusher, Tibshirani, & Chu, 2001) was used to identify differentially expressed genes among different groups using a false discovery rate of less than 10% and fold fold-change >2 as criteria.
|
| |
|
| Submission date |
Jul 12, 2012 |
| Last update date |
Oct 18, 2012 |
| Contact name |
Ruth T Yu |
| Organization name |
Salk Institute
|
| Department |
Gene Expression Lab
|
| Lab |
Ronald Evans
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE39313 |
The role of FGF21 in aging and aging-related diseases |
|
