|
| Status |
Public on Feb 10, 2006 |
| Title |
MamGland normal dietary fat replicate1 PD12 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary Gland
|
| Organism |
Mus musculus |
| Characteristics |
Wild type, Pregnant Day 12 whole mammary gland less Lymph node
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Excised tissue was stored in RNALater and then polytron homogenized and lysed as individual samples in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
| Label |
ENZO biotin labeled
|
| Label protocol |
Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Wildtype FVB mouse mammary tissue, normal dietary fat
|
| Data processing |
GeneSpring version 7.2 GC-RMA processing
|
| |
|
| Submission date |
Feb 08, 2006 |
| Last update date |
Jun 06, 2022 |
| Contact name |
Michael C. Rudolph |
| E-mail(s) |
[email protected]
|
| Organization name |
OUHSC
|
| Department |
Physiology
|
| Lab |
Rudolph Lab
|
| Street address |
975 NE 10th St. BRC North rm 364
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE4222 |
Metabolic regulation in the lactating mammary gland:A lipid synthesizing machine |
|
