Skeletal muscle biopsies were taken from the vastus lateralis before (baseline) or 3H after 75 min of high-intensity cycilng. Total RNA was extracted and amplified using a MessageAmp aRNA Linear Amplification Kit. Amplified RNA was converted to cDNA, labeled with either Cy3 or Cy5 and hybridized to our arrays (Genisphere, Kit 350RP). For each array, we used 976 buffer controls to differentiate signal from noise. Chips were scanned using a Packard Bioscience scanner (Hewlett Packard), ScanArray Express software (version 1.1), a PMT laser setting of 75, and power of 80.
Data processing
TIFF files were analyzed using Genepix 4, using median values for each spot. The data was LOWESS-normalized using GeneTraffic software (Iobion).
