|
| Status |
Public on Oct 03, 2012 |
| Title |
WT_Old_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD150+CD48-LSK bone marrow HSCs
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: adult
age: Old
genotype: WT
tissue: bone marow
cell type: CD150+CD48-LSK HSCs
|
| Growth protocol |
None. Freshly isolated bone marrow hematopoietic stem cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to QIAGEN miRNeasy instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to NuGEN Pico protocol from 50 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Mouse Gene 1.0ST Array. GeneChips were washed and stained in the Tecan HS400 Hyb Station.
|
| Scan protocol |
GeneChips were scanned using the Axon GenePix 4000B scanner.
|
| Description |
Gene expression data from old WT BM, biological replicate 3
|
| Data processing |
The data were analyzed with the RMA method implemented by the affy package of R/Bioconductor
|
| |
|
| Submission date |
Jul 22, 2012 |
| Last update date |
Oct 03, 2012 |
| Contact name |
Wei Tong |
| E-mail(s) |
[email protected]
|
| Phone |
267-626-0930
|
| Organization name |
Children's Hospital of Philadelphia, and University of Pennsylvania School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Tong
|
| Street address |
316A Abramson Bldg., 3615 Civic Center Blvd.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19004 |
| Country |
USA |
| |
|
| Platform ID |
GPL13730 |
| Series (1) |
| GSE39553 |
Young and old HSCs from WT and Lnk-/- mice |
|
