|
| Status |
Public on Jul 23, 2014 |
| Title |
ZT16.5_pulsed 4 |
| Sample type |
RNA |
| |
|
| Source name |
head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
treatment protocol: light pulse at ZT 15
|
| Treatment protocol |
After 4 days in LD 12:12, adult files (age 3-5 d) were exposed to 30 min saturating light pulse at ZT 15 (3 hr after lights off), and were snap frozen after 1 hr (Zt 16.5)
|
| Growth protocol |
Flies were maintened at 25deg. C on standard sugar media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from fly heads was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufacturer's protocol
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol
|
| Scan protocol |
Affymetrix protocol.
|
| Data processing |
R/Bioconductor affylmGUI package (version 1.8.0)
|
| |
|
| Submission date |
Jul 23, 2012 |
| Last update date |
Jul 23, 2014 |
| Contact name |
Eran Tauber |
| Organization name |
University of Leicester
|
| Department |
Genetics
|
| Street address |
University Road
|
| City |
Leicester |
| ZIP/Postal code |
LE1 7RH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE39578 |
Expression analysis of circadian light response in Drosophila |
|
