|
| Status |
Public on Apr 01, 2013 |
| Title |
8_221_T_H |
| Sample type |
RNA |
| |
|
| Source name |
Tak1/Caspase8 LPC double knockout mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Tak1/Caspase8 LPC double knockout mice
gender: male
tissue: liver
age: 6 weeks
|
| Growth protocol |
Liver tissue, excised of 6 week old male mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from snap frozen tissue using the Qiagen RNeasy Kit
|
| Label |
biotin
|
| Label protocol |
Samples were amplified and labelled using the Ambion® WT Expression Kit, combined with the GeneChip® WT Terminal Labeling and Controls Kit according to the manufacturers instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute • Transfer 80μl of hyb solution to each array, then tape holes • Hybridize 16 hours at 45° at 60rpm • Fluidics washing is FS450_00007
|
| Scan protocol |
according to Affymetrix protocol - Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
normalization method: RMA; raw expression threshold: 150, dabg p: 0.05; expression data format: log2
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Jun 06, 2013 |
| Contact name |
Bernd Denecke |
| E-mail(s) |
[email protected]
|
| Phone |
+49 241 8089918
|
| Organization name |
RWTH Aachen University
|
| Department |
IZKF Aachen
|
| Street address |
Pauwelsstrasse 30
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE39750 |
Programmed necrosis and apoptosis differentially regulate cancer development and biliary homeostasis in the liver |
|
