|
| Status |
Public on Oct 10, 2012 |
| Title |
HCmel3_ReTx_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
HCmel3_tumour_ACT_relapse_reinjected
|
| Organism |
Mus musculus |
| Characteristics |
adoptive cell therapies (act): ACT_relapse_reinjected
recipient strain: C57BL6_H-2b
hcmel3 strain: HgfxCdk4R24C_C57BL6
material: liquid_nitrogen_frozen_tumour
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent and purified using Rneasy columns (Quiagen). RNA quality was assayed using Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
100ng RNA was converted to biotinylated cRNA using one round of amplification with Illumina Labelling Kit and one round of T7 polymerase.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
excised_tumour_skin
8294816100_F
|
| Data processing |
Variance stabilization and normalisation using vsn2 package from bioconductor in R without background correction.
|
| |
|
| Submission date |
Aug 20, 2012 |
| Last update date |
Oct 10, 2012 |
| Contact name |
Michael Holzel |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Bonn
|
| Department |
Institute of Clinical Chemistry and Clinical Pharmacology
|
| Lab |
RNA Biology
|
| Street address |
Sigmund-Freud Str. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE40213 |
Expression data from transplanted HCmel3 mouse melanomas relapsing to adoptive T-cell therapy in vivo |
|
