|
| Status |
Public on Mar 08, 2013 |
| Title |
C.glabrata_milbe_4 |
| Sample type |
RNA |
| |
|
| Source name |
C.glabrata_milbe
|
| Organism |
Nakaseomyces glabratus |
| Characteristics |
treatment: treated with Milbemycin A3 oxim
|
| Treatment protocol |
Milbemycin A3 oxim was given at a concentration of 10µg/ml for 1 h in RPMI mediun at 35°C under constant shaking. Same conditions were applied to untreated cells (control)
|
| Growth protocol |
log-pase culture grown in RPMI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction using Trizol
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color quick amp labeling kit protocol
|
| |
|
| Hybridization protocol |
Agilent gene expression hybridization kit protocol
|
| Scan protocol |
Agilent Scanning protocol on an Agilent DNA Microarray Scanner G2565BA Other: Arrays were scanned at 5um resolution and 100/10% PMT XDR
|
| Description |
Milbemycin-treated cells
|
| Data processing |
Data were extracted from the images with Feature Extraction (FE) software. Data were normalized using a combined rank consistency filtering with LOWESS intensity normalization. The gene expression values obtained from FE software were imported into GeneSpring 10.0.2 software (Agilent technologies) for preprocessing and data analysis.
|
| |
|
| Submission date |
Aug 20, 2012 |
| Last update date |
Mar 08, 2013 |
| Contact name |
Dominique Sanglard |
| E-mail(s) |
[email protected]
|
| URL |
http://www.chuv.ch/imul
|
| Organization name |
University of Lausanne and University Hospital Center
|
| Street address |
Rue Bugnon 48
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10713 |
| Series (1) |
| GSE40232 |
Milbemycins: more than efflux inhibitors for fungal pathogens |
|
