|
| Status |
Public on Aug 23, 2012 |
| Title |
Telencephalon cKO 3 |
| Sample type |
RNA |
| |
|
| Source name |
E14.5 cKO 3
|
| Organism |
Mus musculus |
| Characteristics |
age: E14.5
gender: male
tissue: dorsal telencephalon
genotype: histone methyltransferase ESET KO
genetic background: C57BL\6
|
| Treatment protocol |
Dorsal Telencephalon of WT or cKO (Emx2Cre) E14.5 embryos were dissected and dissolved in Trizol immediately until RNA extraction is performed.
|
| Growth protocol |
Mice were housed in SPF conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug cRNA were hybridized for 16 hr at 45C on GeneChip Mus Musculus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from embryos at E14.5
|
| Data processing |
The data were analyzed with Expression Console Software using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 22, 2012 |
| Last update date |
Aug 28, 2012 |
| Contact name |
Siok Lay Tan |
| Organization name |
Kyoto University
|
| Street address |
Sakyo-Ku, Shogoin Kawahara
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE40296 |
Essential roles of the histone methyltransferase ESET in the epigenetic control of neural progenitor cells during development |
|
