|
| Status |
Public on Jun 06, 2013 |
| Title |
Wild type rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genetic background: Oregon R
genotype: wild type
tissue: whole embryo
developmental stage: embryonic stages 11-16
|
| Treatment protocol |
Embryos were sorted on a COPAS biosorter prior to RNA extraction.
|
| Growth protocol |
Embryos were collected and aged at 25 C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by TriZol extraction and cleaned up using the Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Amplification and labeling were performed using the Affymetrix GeneChip3' IVT express kit (P/N 901229) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples and controls were prepared using the Affymetrix GeneChip hybridization, wash and stain kit (P/N 900720). Arrays were prehybridized for 10 minutes prior to sample addition. Following sample addition, arrays were hybridized for 16 hours at 45 Celsius, and shaken at 60 rpm. Arrays were stained and washed using the Affymetrix GeneChip Fluidics Station 450 and FS450_002 fluidics script.
|
| Scan protocol |
Arrays were scanned with the Affymetrix GeneChip Scanner 3000 and raw analysis was performed with Affymetrix Command Console and Expression Console.
|
| Data processing |
Data was normalized by RMA and statistical analysis was performed using Partek software. Further analysis was performed using Spotfire software.
|
| |
|
| Submission date |
Aug 24, 2012 |
| Last update date |
Jun 06, 2013 |
| Contact name |
Deborah J Andrew |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Cell Biology
|
| Street address |
725 N. Wolfe St., Hunterian G-1
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE40358 |
Gene regulated by ectopic expression of Sage in the entire Drosophila embryo |
|
