tissue: brain, Right Frontal Lobe histology: GBM tumor number (as appears in figure 3): 712 age: 49 gender: MALE grade: 4 living: DECEASED survival time: 188 survival cluster: SC2 hc_74_samples: HC2B hc_85_samples: HC2B array: 133B
Extracted molecule
total RNA
Extraction protocol
RNA extraction was performed as described previously (Mischel PS, Shai R, Shi T, et al Identification of molecular subtypes of glioblastoma by gene expression profiling. Oncogene, 2003;22:2361-73) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA extraction was performed as described previously (Mischel PS, Shai R, Shi T, et al Identification of molecular subtypes of glioblastoma by gene expression profiling. Oncogene, 2003;22:2361-73) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Label protocol
Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A and HG-U133B arrays (Affymetrix, Santa Clara, CA). Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A and HG-U133B arrays (Affymetrix, Santa Clara, CA).
Hybridization protocol
Standard Affymetrix protocols were used. Standard Affymetrix protocols were used.
Scan protocol
Standard Affymetrix protocols were used. Standard Affymetrix protocols were used.
