tissue: brain, Left frontal Lobe histology: GBM tumor number (as appears in figure 3): 1478 age: 40 gender: MALE grade: 4 living: ALIVE survival time: 1088 survival cluster: SC1 hc_74_samples: HC1A hc_85_samples: HC1A array: 133B
Extracted molecule
total RNA
Extraction protocol
RNA extraction was performed as described previously (Mischel PS, Shai R, Shi T, et al Identification of molecular subtypes of glioblastoma by gene expression profiling. Oncogene, 2003;22:2361-73) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Label protocol
Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A and HG-U133B arrays (Affymetrix, Santa Clara, CA).
