GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM99621

Query DataSets for GSM99621

Status

Public on Mar 12, 2006

Title

208F control

Sample type

RNA

Source name

208Fimmortal rat fibroblast

Organism

Rattus norvegicus

Characteristics

cultured cells

Extracted molecule

total RNA

Extraction protocol

Total RNA was prepared from cultured cells using the TRIzol Reagent (Invitrogen) according to the manufacturer's instructions and dissolved in nuclease free water. After isolation, RNA used for microarray hybridisations was purified by the RNeasy Mini Kit (Qiagen) and the RNA quality was determined on a LabChip Bioanalyzer (Agilent Technologies).

Label

biotin

Label protocol

Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

208F immortal fibroblasts untreated

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Mar 08, 2006

Last update date

Mar 09, 2006

Contact name

Christine Sers

E-mail(s)

[email protected]

Organization name

Charite

Department

Institute of Pathology

Street address

Schumannstr. 20/21

City

Berlin

ZIP/Postal code

10117

Country

Germany

Platform ID

GPL85

Series (1)

GSE4427

Methylation induced by the RAS oncogene

Data table header descriptions
ID_REF
VALUE	calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A03913cds_s_at	109	P	0.031336
A30543cds_s_at	93	A	0.378184
A44407cds_at	153	P	0.035163
AA108277_at	589	P	0.000673
AA108308_s_at	532	A	0.117160
AA684537_at	391	P	0.035163
AA684929_f_at	213	A	0.204022
AA684960_f_at	125	A	0.541184
AA684963_at	1193	P	0.000673
AA685112_at	829	P	0.001892
AA685152_r_at	183	P	0.023926
AA685903_at	4204	P	0.000219
AA686031_at	197	P	0.013092
AA686579_at	243	P	0.000959
AA686743_at	37	A	0.250724
AA686870_f_at	27	A	0.679170
AA686870_i_at	42	A	0.533936
AA799276_at	744	P	0.019304
AA799336_at	613	P	0.000219
AA799389_g_at	66	A	0.320830

Total number of rows: 4847

Table truncated, full table size 135 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM99621.DAT.gz	27.3 Mb	(ftp)(http)	DAT
GSM99621.EXP.gz	491 b	(ftp)(http)	EXP