|
| Status |
Public on Nov 29, 2012 |
| Title |
H9-derived oligodendrocytes technical rep 1-2-2 |
| Sample type |
RNA |
| |
|
| Source name |
Oligos O4+ from H9
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H9
cell type: oligodendrocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX ref8 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Slides were scanned using Illumina BeadArray Reader scanner
|
| Description |
Gene expression data from H9 oligos
M2-bis
|
| Data processing |
The data were analyzed with GenomeStudio using Illumina default analysis settings. Quantile normalization is performed.
|
| |
|
| Submission date |
Sep 04, 2012 |
| Last update date |
Nov 29, 2012 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE40593 |
Impaired intrinsic immunity to HSV-1 in human iPSC-derived TLR3-deficient CNS cells |
|
