|
Status |
Public on Nov 29, 2012 |
Title |
H9-derived neurons CD44- EGFR- biological rep 1-3 |
Sample type |
RNA |
|
|
Source name |
Neurons CD44- EGFR- from H9
|
Organism |
Homo sapiens |
Characteristics |
cell line: H9 cell type: neurons CD44- EGFR-
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX ref8 Array. Slides were washed and stained in the Illumina instrument.
|
Scan protocol |
Slides were scanned using Illumina BeadArray Reader scanner
|
Description |
Gene expression data from H9 neurons FL3
|
Data processing |
The data were analyzed with GenomeStudio using Illumina default analysis settings. Quantile normalization is performed.
|
|
|
Submission date |
Sep 04, 2012 |
Last update date |
Nov 29, 2012 |
Contact name |
Jeffrey Zhao |
Organization name |
Memorial Sloan Kettering Cancer Center
|
Department |
Genomics Core
|
Street address |
1275 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE40593 |
Impaired intrinsic immunity to HSV-1 in human iPSC-derived TLR3-deficient CNS cells |
|