|
| Status |
Public on May 01, 2013 |
| Title |
S63del sciatic nerve at P28, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Sciatic nerves from P28 S63del mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Sciatic nerve
genotype: P0S63del
age: Postnatal day 28
|
| Treatment protocol |
Sciatic nerves were dissected, the perineureal sheath was removed, and the nerve were snap-frozen with liquid nitrogen. Prior to RNA extraction, the nerves were pulverized with mortar and pestle, and transferred in the Trizol reagent (Invitrogen).
|
| Growth protocol |
Sciatic nerves were dissected from WT, CHOP null, S63del amd S63del/CHOP null mice at the indicated timepoints (P5, P28 and 4-months)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was then purified with Rneasy kit (Qiagen) following manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression from P28 S63del (neuropathic) scatic nerve
|
| Data processing |
Data were analysed with GeneChip Operating Software v1.4 (GCOS1.4).
|
| |
|
| Submission date |
Sep 05, 2012 |
| Last update date |
May 01, 2013 |
| Contact name |
Maurizio D'Antonio |
| E-mail(s) |
[email protected]
|
| Organization name |
San Raffaele Scientific Institute
|
| Department |
DIBIT
|
| Street address |
via Olgettina 58
|
| City |
Milano |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE40610 |
Expression data from a Charcot-Marie-Tooth 1B neuropathy mouse model |
|
