LOCUS NM_001321103 8079 bp mRNA linear PRI 23-APR-2024
DEFINITION Homo sapiens solute carrier family 4 member 7 (SLC4A7), transcript
variant 4, mRNA.
ACCESSION NM_001321103 XM_011534255
VERSION NM_001321103.2
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 8079)
AUTHORS Axelsen,T.V., Olesen,C., Khan,D., Mohammadi,A., Bouzinova,E.V.,
Nielsen,C.J.F., Mele,M., Hauerslev,K.R., Pedersen,H.L., Balling,E.,
Vahl,P., Tramm,T., Christiansen,P.M. and Boedtkjer,E.
TITLE Antibodies toward Na+,HCO3--cotransporter NBCn1/SLC4A7 block net
acid extrusion and cause pH-dependent growth inhibition and
apoptosis in breast cancer
JOURNAL Br J Cancer 130 (7), 1206-1220 (2024)
PUBMED 38310186
REMARK GeneRIF: Antibodies toward Na[+],HCO3[-]-cotransporter NBCn1/SLC4A7
block net acid extrusion and cause pH-dependent growth inhibition
and apoptosis in breast cancer.
REFERENCE 2 (bases 1 to 8079)
AUTHORS Severin,M., Pedersen,E.L., Borre,M.T., Axholm,I.,
Christiansen,F.B., Ponniah,M., Czaplinska,D., Larsen,T., Pardo,L.A.
and Pedersen,S.F.
TITLE Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the
plasma membrane, centrosomes, spindle and primary cilia
JOURNAL J Cell Sci 136 (7) (2023)
PUBMED 37039101
REMARK GeneRIF: Dynamic localization of the Na+-HCO3- co-transporter NBCn1
to the plasma membrane, centrosomes, spindle and primary cilia.
REFERENCE 3 (bases 1 to 8079)
AUTHORS Zhou,Y., Ma,X. and Sun,J.
TITLE Update on the relationship between the SLC4A7 variant rs4973768 and
breast cancer risk: a systematic review and meta-analysis
JOURNAL J Int Med Res 51 (4), 3000605231166517 (2023)
PUBMED 37128157
REMARK GeneRIF: Update on the relationship between the SLC4A7 variant
rs4973768 and breast cancer risk: a systematic review and
meta-analysis.
REFERENCE 4 (bases 1 to 8079)
AUTHORS Ali,E.S., Liponska,A., O'Hara,B.P., Amici,D.R., Torno,M.D., Gao,P.,
Asara,J.M., Yap,M.F., Mendillo,M.L. and Ben-Sahra,I.
TITLE The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de
novo nucleotide synthesis
JOURNAL Mol Cell 82 (17), 3284-3298 (2022)
PUBMED 35772404
REMARK GeneRIF: The mTORC1-SLC4A7 axis stimulates bicarbonate import to
enhance de novo nucleotide synthesis.
REFERENCE 5 (bases 1 to 8079)
AUTHORS Pushkin,A., Abuladze,N., Newman,D., Muronets,V., Sassani,P.,
Tatishchev,S. and Kurtz,I.
TITLE The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ
motifs involved in their interaction
JOURNAL Am J Physiol Cell Physiol 284 (3), C667-C673 (2003)
PUBMED 12444018
REFERENCE 6 (bases 1 to 8079)
AUTHORS Park,M., Ko,S.B., Choi,J.Y., Muallem,G., Thomas,P.J., Pushkin,A.,
Lee,M.S., Kim,J.Y., Lee,M.G., Muallem,S. and Kurtz,I.
TITLE The cystic fibrosis transmembrane conductance regulator interacts
with and regulates the activity of the HCO3- salvage transporter
human Na+-HCO3- cotransport isoform 3
JOURNAL J Biol Chem 277 (52), 50503-50509 (2002)
PUBMED 12403779
REMARK GeneRIF: The cystic fibrosis transmembrane conductance regulator
interacts with and regulates the activity of the bicarbonate
salvage trransporter isoform 3
REFERENCE 7 (bases 1 to 8079)
AUTHORS Pushkin,A., Yip,K.P., Clark,I., Abuladze,N., Kwon,T.H.,
Tsuruoka,S., Schwartz,G.J., Nielsen,S. and Kurtz,I.
TITLE NBC3 expression in rabbit collecting duct: colocalization with
vacuolar H+-ATPase
JOURNAL Am J Physiol 277 (6), F974-F981 (1999)
PUBMED 10600945
REFERENCE 8 (bases 1 to 8079)
AUTHORS Pushkin,A., Abuladze,N., Lee,I., Newman,D., Hwang,J. and Kurtz,I.
TITLE Cloning, tissue distribution, genomic organization, and functional
characterization of NBC3, a new member of the sodium bicarbonate
cotransporter family
JOURNAL J Biol Chem 274 (23), 16569-16575 (1999)
PUBMED 10347222
REFERENCE 9 (bases 1 to 8079)
AUTHORS Pushkin,A., Abuladze,N., Lee,I., Newman,D., Hwang,J. and Kurtz,I.
TITLE Mapping of the human NBC3 (SLC4A7) gene to chromosome 3p22
JOURNAL Genomics 57 (2), 321-322 (1999)
PUBMED 10198178
REFERENCE 10 (bases 1 to 8079)
AUTHORS Ishibashi,K., Sasaki,S. and Marumo,F.
TITLE Molecular cloning of a new sodium bicarbonate cotransporter cDNA
from human retina
JOURNAL Biochem Biophys Res Commun 246 (2), 535-538 (1998)
PUBMED 9610397
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AK309734.1, EU934248.1,
EU934249.1, FJ178574.1, EU499349.1, AK310865.1, AF053755.1,
AF047033.1, AL389947.1 and AA569234.1.
This sequence is a reference standard in the RefSeqGene project.
On Jun 2, 2019 this sequence version replaced NM_001321103.1.
Summary: This locus encodes a sodium bicarbonate cotransporter. The
encoded transmembrane protein appears to transport sodium and
bicarbonate ions in a 1:1 ratio, and is thus considered an
electroneutral cotransporter. The encoded protein likely plays a
critical role in regulation of intracellular pH involved in visual
and auditory sensory transmission. Alternatively spliced transcript
variants encoding distinct isoforms have been described. [provided
by RefSeq, Apr 2012].
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: EU934248.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000454389.6/ ENSP00000390394.1
RefSeq Select criteria :: based on manual assertion, conservation,
longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-37 AK309734.1 37-73
38-494 EU934248.1 1-457
495-997 EU934249.1 446-948
998-1130 EU934248.1 961-1093
1131-1408 FJ178574.1 1043-1320
1409-2603 EU934249.1 988-2182
2604-2604 EU499349.1 2183-2183
2605-3773 EU934249.1 2184-3352
3774-3774 AK310865.1 132-132
3775-4196 EU934248.1 3738-4159
4197-5412 AF053755.1 3181-4396
5413-6279 AF047033.1 5092-5958
6280-8078 AL389947.1 668-2466
8079-8079 AA569234.1 11-11 c
FEATURES Location/Qualifiers
source 1..8079
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3p24.1"
gene 1..8079
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="solute carrier family 4 member 7"
/db_xref="GeneID:9497"
/db_xref="HGNC:HGNC:11033"
/db_xref="MIM:603353"
exon 1..318
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
misc_feature 229..231
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="upstream in-frame stop codon"
CDS 259..4038
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="isoform d is encoded by transcript variant 4;
sodium bicarbonate cotransporter 2; sodium bicarbonate
cotransporter 3; electroneutral Na/HCO(3) cotransporter;
bicarbonate transporter; sodium bicarbonate cotransporter
2b; solute carrier family 4, sodium bicarbonate
cotransporter, member 7"
/codon_start=1
/product="sodium bicarbonate cotransporter 3 isoform d"
/protein_id="NP_001308032.1"
/db_xref="CCDS:CCDS82747.1"
/db_xref="GeneID:9497"
/db_xref="HGNC:HGNC:11033"
/db_xref="MIM:603353"
/translation="MEADGAGEQMRPLLTRVTSRGPDEEAVVDLGKTSSTVNTKFEKE
ELESHRAVYIGVHVPFSKESRRRHRHRGHKHHHRRRKDKESDKEDGRESPSYDTPSQR
VQFILGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARWLKFEEDVEDGGDRWSK
PYVATLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDESIRENVR
EALLKRHHHQNEKRFTSRIPLVRSFADIGKKHSDPHLLERNGEGLSASRHSLRTGLSA
SNLSLRGESPLSLLLGHLLPSSRAGTPAGSRCTTPVPTPQNSPPSSPSISRLTSRSSQ
ESQRQAPELLVSPASDDIPTVVIHPPEEDLEAALKGEEQKNEENVDLTPGILASPQSA
PGNLDNSKSGEIKGNGSGGSRENSTVDFSKVDMNFMRKIPTGAEASNVLVGEVDFLER
PIIAFVRLAPAVLLTGLTEVPVPTRFLFLLLGPAGKAPQYHEIGRSIATLMTDEIFHD
VAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPVFHNGST
PTLGETPKEAAHHAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFL
YCACMSPVITFGGLLGEATEGRISAIESLFGASLTGIAYSLFAGQPLTILGSTGPVLV
FEKILYKFCRDYQLSYLSLRTSIGLWTSFLCIVLVATDASSLVCYITRFTEEAFAALI
CIIFIYEALEKLFDLGETYAFNMHNNLDKLTSYSCVCTEPPNPSNETLAQWKKDNITA
HNISWRNLTVSECKKLRGVFLGSACGHHGPYIPDVLFWCVILFFTTFFLSSFLKQFKT
KRYFPTKVRSTISDFAVFLTIVIMVTIDYLVGVPSPKLHVPEKFEPTHPERGWIISPL
GDNPWWTLLIAAIPALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVGVMLG
VCSVMGLPWFVAATVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFILMGL
SVFMTSVLKFIPMPVLYGVFLYMGVSSLKGIQLFDRIKLFGMPAKHQPDLIYLRYVPL
WKVHIFTVIQLTCLVLLWVIKVSAAAVVFPMMVLALVFVRKLMDLCFTKRELSWLDDL
MPESKKKKEDDKKKKEKEEAERMLQDDDDTVHLPFEGGSLLQIPVKALKYSVDPSIVN
ISDEMAKTAQWKALSMNTENAKVTRSNMSPDKPVSVKISFEDEPRKKYVDAETSL"
exon 319..400
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 401..547
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 548..686
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 687..847
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 848..1036
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1037..1408
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1409..1524
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1525..1682
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1683..1770
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1771..1917
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 1918..2024
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2025..2199
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2200..2333
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2334..2579
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2580..2685
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2686..2847
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2848..2961
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 2962..3123
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3124..3375
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3376..3444
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3445..3618
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3619..3750
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3751..3848
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3849..3956
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
exon 3957..8079
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/inference="alignment:Splign:2.1.0"
regulatory 7285..7290
/regulatory_class="polyA_signal_sequence"
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="hexamer: ATTAAA"
polyA_site 7308
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="major polyA site"
regulatory 8060..8065
/regulatory_class="polyA_signal_sequence"
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="hexamer: AATAAA"
polyA_site 8079
/gene="SLC4A7"
/gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
/note="major polyA site"
ORIGIN
1 agttctagct tcaggttcca cgccgcggcc tcgctctcct actaaagcca gcccagcagt
61 cgcagcgcgc acacgcacgc cccgcgtctc ccgcgcccgg cgcccggctc tccccacgcg
121 cgcacacctc gcgcacggac gcacccacgt ttgtcctcgc gcgccggggg ctcggcggcg
181 gcggcagggg cagcggcaga agcaggcaga ccgcgcgggg cagtaccgta gcggccgtca
241 cgggctggcc ggccggccat ggaggctgat ggggccggcg agcagatgag accgctactc
301 acccgggtaa cgagcagggg tcctgatgaa gaagctgttg tggatcttgg caaaactagc
361 tcaactgtga acaccaagtt tgaaaaagaa gaactagaaa gtcatagagc tgtatatatt
421 ggtgttcacg tcccgtttag taaagagagt cgtcggcgtc ataggcatcg cggacacaaa
481 catcaccacc ggagaagaaa agataaagaa tcagataaag aagatggacg ggaatctcct
541 tcttatgata caccatccca gagagttcag tttatccttg gtactgaaga tgatgatgaa
601 gaacatattc cccatgatct cttcacggaa atggatgaac tgtgttacag agatggagaa
661 gaatatgaat ggaaagaaac tgctagatgg ctgaaatttg aagaggatgt tgaagatggc
721 ggtgaccgat ggagtaaacc ttatgtggca actctctctt tgcacagtct ttttgaacta
781 aggagttgca tcctcaatgg aacagtcatg ctggatatga gagcaagcac tctagatgaa
841 atagcagata tggtattaga caacatgata gcttctggcc aattagacga gtccatacga
901 gagaatgtca gagaagctct tctgaagaga catcatcatc agaatgagaa aagattcacc
961 agtcggattc ctcttgttcg atcttttgca gatataggca agaaacattc tgaccctcac
1021 ttgcttgaaa ggaatgggga aggcctttca gcctcccgcc actctttgcg aacaggtctg
1081 tctgcctcaa acctttcctt gagaggagaa tcacctttat ctcttcttct tggtcatctt
1141 cttccttctt caagagctgg aacccctgca ggctcaaggt gtacaacccc agtacccacc
1201 cctcaaaaca gtcctccttc tagccctagc atcagccgcc tgacctccag aagttcccaa
1261 gagagtcagc gtcaggcccc agaactactg gtttcacctg ccagtgatga tattcccaca
1321 gtagtaattc atccgcctga ggaagactta gaagcagcgc tgaaaggcga ggagcagaag
1381 aatgaggaaa atgttgactt aactccaggt attttggcct ctccccagtc tgctcctgga
1441 aacttggaca atagtaaaag tggagaaatt aaaggtaatg gaagtggtgg aagcagagaa
1501 aatagtactg ttgacttcag caaggttgat atgaatttca tgagaaaaat tcctacgggt
1561 gctgaggcat ccaacgtcct ggtgggcgaa gtagactttt tggaaaggcc aataattgca
1621 tttgtgagac tggctcctgc tgtcctcctt acagggttga ctgaggtccc tgttccaacc
1681 aggtttttgt ttttgttatt gggtccagcg ggcaaggcac cacagtacca tgaaattgga
1741 cgatcaatag ccactctcat gacagatgag attttccatg atgtagctta taaagcaaaa
1801 gacagaaatg acctcttatc tggaattgat gaatttttag atcaagtaac tgtcctacct
1861 ccaggagagt gggatccttc tatacgcata gaaccaccaa aaagtgtccc ttctcaggaa
1921 aagagaaaga ttcctgtgtt tcacaatgga tctaccccca cactgggtga gactcctaaa
1981 gaggccgctc atcatgctgg gcctgagcta cagaggactg gacggctttt tggtggtttg
2041 atacttgaca tcaaaaggaa agcacctttt ttcttgagtg acttcaagga tgcattaagc
2101 ctgcagtgcc tggcctcgat tcttttccta tactgtgcct gtatgtctcc tgtaatcact
2161 tttggagggc tgcttggaga agctacagaa ggcagaataa gtgcaataga gtctcttttt
2221 ggagcatcat taactgggat tgcctattca ttgtttgctg ggcaacctct aacaatattg
2281 gggagcacag gtccagttct agtgtttgaa aaaattttat ataaattctg cagagattat
2341 caactttctt atctgtcttt aagaaccagt attggtctgt ggacttcttt tttgtgcatt
2401 gttttggttg caacagatgc aagcagcctt gtgtgttata ttactcgatt tacagaagag
2461 gcttttgcag cccttatttg catcatattc atctacgagg ctttggagaa gctctttgat
2521 ttaggagaaa catatgcatt taatatgcac aacaacttag ataaactgac cagctactca
2581 tgtgtatgta ctgaacctcc aaaccccagc aatgaaactc tagcacaatg gaagaaagat
2641 aatataacag cacacaatat ttcctggaga aatcttactg tttctgaatg taaaaaactt
2701 cgtggtgtat tcttggggtc agcttgtggt catcatggac cttatattcc agatgtgctc
2761 ttttggtgtg tcatcttgtt tttcacaaca ttttttctgt cttcattcct caagcaattt
2821 aagaccaagc gttactttcc taccaaggtg cgatcgacaa tcagtgattt tgctgtattt
2881 ctcacaatag taataatggt tacaattgac taccttgtag gagttccatc tcctaaactt
2941 catgttcctg aaaaatttga gcctactcat ccagagagag ggtggatcat aagcccactg
3001 ggagataatc cttggtggac cttattaata gctgctattc ctgctttgct ttgtaccatt
3061 ctcatcttta tggatcaaca aatcacagct gtaattataa acagaaagga acacaaattg
3121 aagaaaggag ctggctatca ccttgatttg ctcatggttg gcgttatgtt gggagtttgc
3181 tctgtcatgg gacttccatg gtttgtggct gcaacagtgt tgtcaataag tcatgtcaac
3241 agcttaaaag ttgaatctga atgttctgct ccaggggaac aacccaagtt tttgggaatt
3301 cgtgaacagc gggttacagg gctaatgatt tttattctaa tgggcctctc tgtgttcatg
3361 acttcagtcc taaagtttat tccaatgcct gttctgtatg gtgttttcct ttatatggga
3421 gtttcctcat taaaaggaat ccagttattt gaccgtataa aattatttgg aatgcctgct
3481 aagcatcagc ctgatttgat atacctccgt tatgtgccgc tctggaaggt ccatattttc
3541 acagtcattc agcttacttg tttggtcctt ttatgggtga taaaagtttc agctgctgca
3601 gtggtttttc ccatgatggt tcttgcatta gtgtttgtgc gcaaactcat ggacctgtgt
3661 ttcacgaaga gagaacttag ttggcttgat gatcttatgc cagaaagtaa gaaaaagaaa
3721 gaagatgaca aaaagaaaaa agagaaagag gaagctgaac ggatgcttca agatgatgat
3781 gatactgtgc accttccatt tgaaggggga agtctcttgc aaattccagt caaggcccta
3841 aaatatagtg ttgatccctc aattgttaac atatcagatg aaatggccaa aactgcacag
3901 tggaaggcac tttccatgaa tactgagaat gccaaagtaa ccagatctaa catgagtcct
3961 gataaacctg tgagtgtgaa aataagtttt gaagatgaac caagaaagaa atacgtggat
4021 gctgaaactt cattatagaa ttgaaccaag aggcattata catatagata tatacatatg
4081 taatgtgtgc gtatcatgtc actatatata agaatattgt atgtcatgct gtttatgtgt
4141 gactaccggg tttttaaaag tagtgtctgg agtttgtaat gagcaccgtg gagactatgt
4201 atttaatgaa atgctctctt tgaagtgagg tacatggttc ttaactattc aaatatttat
4261 tctgttagaa aaaaaaattt tctgttttgc aatagaagga tgtggagaaa tgctttcagt
4321 ctacttttct taaatctctg ttcatcagtg gcaattcgta aaaaccttaa gtgatacttt
4381 gtttatatgt ttataatttt taggtgtttc ctgaaatttt cacatattat ttcacttttg
4441 ttagtgcttt atgggaagaa tagggagtct ataccagtgc tgtgggaaaa atggtaacat
4501 ttcagggctt ctctatttgt gtttcatttc tgtagatgtc catcgtgttt cactaactgg
4561 cgttttctta gccatagaga tgactgtaga acaatagaaa ctttaataat gatagttttt
4621 aacttttatg tttaaatttt ttttaaatct taaaaccttc atatctaggt atccattgtg
4681 acagacaagt aaaattgcag gtgatttgat aattaagcac ccataccatt tataacttct
4741 gaatttaaaa agttatacaa tgccagtttg caatagttga ttttgatgcc tttgtagaat
4801 attttttctg aatccttatg ctcttttaaa gccaatgatt cccactctgt tctctgcctt
4861 gtctctcttt gtcttaaaat gctttagttt ccatcaggtt caagttcttg actattattc
4921 tcttataagt agtaggcgta aataatcagg agttagaatt ctctcagaag ggtctatgat
4981 cagtattact ttattaagaa ttacctttca ttttctcttt atgttattct ttcacttttg
5041 tagattacat ttaatagctt gttacctgtg attttatttt aaaatattta ttttgatatg
5101 atgcttaaat attatataaa catttggaaa agtaacaaat agagtaaatt gttaatgtaa
5161 atagttggtg cttactttca tttatgttta ttatcttaaa gcaattgata gattttacat
5221 ctttgatata aagcactgcc atatttatat tttaaaagga aattagacat tttatatgta
5281 gctcagatta atgacatttt tattttgtgt attagttttt gcttttctga gctttttaaa
5341 gtctaacaaa tctttttagt catcttttat atacttttag ttccacatga aataaatgtt
5401 gttaagcctg taggactgga tgaatggggt tgtgaaactg atttgacaag agaataattt
5461 acaaaaatca aatgagtatt gagaagccac agaaatatca aaaatggtga catcactatt
5521 aggatgaaaa ttttatgaaa ttccaatgct ccctttaacc atataattaa attacaaagg
5581 tatacataat taatctaata aaggatcatg aggaatgcca aaagctgaaa ttttagcaag
5641 tctggtgttt taaattcatt ataaactatt tattaactaa tgtaatgtcc tcctctaaga
5701 ccatattgta agagtcttgt taaaatgatg gattcaactt ctgcctcgga tgagaggttg
5761 gaaatgtagc tgcttttctt tagaaaatat gtagctgtca tcatttggta ctgcctaaaa
5821 agagttagac tctattggca attgatagag tattacccac ggtgcttatt attgtttatg
5881 agcttccatt gtaatgattc ctttttattt gtagcagcat aattatttcc aaagacccca
5941 gtatttgctg ctatttttaa aactcccctg atgtacctga acaagaggat ttcctcacat
6001 catttccttg tgtctggaca tcaggggtaa caactgtact tactttacag gaagaaattt
6061 taaaactgaa aactacctgg gatcatagtg tttctgtgat tttatttaat tgtgtatagt
6121 aattatccag tgccagaaaa accgtcactt gcaaaatact ttgcactcaa aatgttttta
6181 caatgcttct aaatgttact ggtttctgct ttcttttgac tacttgactg acaaaatgat
6241 ctgactactc catttaagag caaaggtaac tcatgtttag gtaattaact gcttgtttta
6301 agtgattata tttcttccac tgtttttgaa aaataatcaa agatagcatt cattgagaga
6361 cagtgacaga tataatttac tacatatatt gatttttaaa taaagttgcc ttaaataagt
6421 gtatgtaagc agtagtagtt gctatgtact gatttacctc aaggtgcaaa ataattaaac
6481 ctgtacatat tccatttaca aaataaattc agccctgcac tttctttaga tgccttgatt
6541 tccagaatgg agcttagtgc tactgaatac cctggccaca gagccacctc aggatattct
6601 tttctccacc ctagtttatt tatttataga tatctgttta caaagtctgt agtaaatcct
6661 gatgctgacc atctgaaatg tacttttttt ctgaatgctg tttcaatcta aaatagcagc
6721 ttttgagaaa acaatgatgt aaattcctta tgataaaagg atgattctat atattcttta
6781 atgatattaa atatgccgaa gccaagcaca cagtctttct aaagtgtgtg tatgtttgtg
6841 tgaatgtgaa tgatactgat cttatatctg ttaaaagttg ttttaaaaag ctgtggcatc
6901 ccattgttca tatttgccaa gtcttctgta aagatgtcta ggacgaaata ttttatgtgc
6961 taatgcatgt atttgtaaac cagatttgtt taccactcaa aattaacttg ttttcttcat
7021 ccaaaaaagt ttatttcttc cacgtactta aattttctgt gtgggtataa tatagctttc
7081 taattttttt ctttcacaaa ggcaggttca aaattctgtt gaaagaaaaa tgctttctga
7141 aactgaggta taacaccaga gcttgctgtt taaaggatta tatgatgtac atcagttcta
7201 taaatgtgct cagcagttta acatgtgaat cctgttttaa agtgctcaga tttcaactgt
7261 gtaagccatt gatataacgc tgtaattaaa aatgtttata tgaaataact taatgtttta
7321 aatttattta tgtagatcac atcattttta tcgtatgcag tgcaaatatg tgaaatgtct
7381 tttggtttat tccaacaatt atttatttta gaaagtaagt ttaaagactt taaggacatt
7441 caaagtttaa aatagtgttc aaattgcaaa atttggcaat cttcatataa attggtttct
7501 tttctaactt ttcaaaaact aacattaaat gtcaattata ggaaaacata gttggaaatg
7561 taatcatcca aagatcattt ttaaaatgaa atttaattag cacatattga acatttgact
7621 taattgttaa accccagttt tgttttgttt ttttaatcag atttttgcac actgattagt
7681 ttttgtgttg tggcttttgt tgctttatta ttcaaggttt tttttttttt tcttccccat
7741 gggggagatt gtcttccaat gtttaactac gtttaaataa ataaaaattg aattttattg
7801 ttcatttata taaaatctga taccttgatg taatttcaca atacagttcc aatttttatg
7861 gcttttataa ttacaatgat attttcttct ataataaaat ccaaagtaaa catttaaatt
7921 gtagaactga tatttttcat ttatatgaag tataagcctc tactgggtct atattgtgaa
7981 tcatcctgcc tttcaaattt gtttcataat tgttagatga aaactatttt tttggagatg
8041 ttactgaagt tgattgagca ataaaagtct acttaatta
//