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SRX8357296: Human fecal metatranscriptome from 10 mM sulfoquinovose-amended microcosm after 20 h incubation. Replicate microcosm 1
1 ILLUMINA (Illumina HiSeq 3000) run: 28M spots, 1.4G bases, 483.1Mb downloads

Design: Total nucleic acids were extracted from microcosm biomass using bead-beating with phenol/chloroform (pH8). Resultant RNA pools after Dnase digestion were subject to rRNA substratction and used for metatranscriptomic library preparation.
Submitted by: University of Vienna
Study: Sulfoquinovose is a selective nutrient for bacteria in the human gut
show Abstracthide Abstract
Exploration of sulfoquinovose degradation by the human gut microbiota. Homogenized human feces from 8 vegetarians was used to construct microcosms, which were amended with sulfoquinovose and controls (e.g. glucose, taurine, no amendment, etc.). Microcosms were subsampled and extracted nucleic acids were screened using amplicon sequencing (16S rRNA gene, dsrB), metagenomics, and metatranscriptomics.
Sample: Human fecal metatranscriptome from 10 mM sulfoquinovose-amended microcosm after 20 h incubation replicate 1
SAMN14943933 • SRS6673415 • All experiments • All runs
Library:
Name: SQ_HumanFecalMicrocosmT20h_metatranscriptome_replicate_1
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: METATRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Runs: 1 run, 28M spots, 1.4G bases, 483.1Mb
Run# of Spots# of BasesSizePublished
SRR1180586628,028,7901.4G483.1Mb2020-05-29

ID:
10870261

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