GSM7061235: Primary Lung fibroblast, Negative control, biol rep 1; Ho... - SRA

SRX19487913: GSM7061235: Primary Lung fibroblast, Negative control, biol rep 1; Homo sapiens; RNA-Seq
4 ILLUMINA (NextSeq 550) runs: 20.7M spots, 3.1G bases, 1.3Gb downloads

External Id: GSM7061235_r1

Submitted by: Severance Biomedical Science Institute, Yonsei University College of Medicine

Study: Effect of ezetimibe treatment on gene expression of TGFß1-activated primary human lung fibroblasts

PRJNA938140 • SRP424268 • All experiments • All runs

show Abstracthide Abstract

To verify whether ezetimibe inhibits myofibroblast differentiation at the transcriptome level and to reveal the mechanism of action of ezetimibe, we sequenced primary human lung fibroblasts that we isolated and cultured. Overall design: Comparative gene expression analysis of mRNA-Seq data from primary human lung fibroblasts treated with TGFß1 and/or ezetimibe (Negative control/ TGFß1-treated positive control / TGFß1&ezetimibe-treated group).

Sample: Primary Lung fibroblast, Negative control, biol rep 1

SAMN33426543 • SRS16878826 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7061235

Instrument: NextSeq 550

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was harvested using Rneasy mini kit (QIAGEN). RNA libraries for mRNA (polyA RNA) sequencing were prepared using Illumina stranded mRNA prep kit following the manufacturer's protocols.

Runs: 4 runs, 20.7M spots, 3.1G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR23603013	5,152,437	762.6M	324.2Mb	2024-02-23
SRR23603014	5,025,712	743.8M	319Mb	2024-02-23
SRR23603015	5,351,131	792M	334.6Mb	2024-02-23
SRR23603016	5,184,572	767.3M	327.2Mb	2024-02-23

ID:: 26767859

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